In vitro Regeneration and Propagation of Chickpea (Cicer ...

Regeneration & Genetic Transformation in Chickpea (Cicer arietinum L.): A Non-Conventional Approach

The J. Anim. Plant Sci. 27(6): 2017 SYSTEMS IN LOCAL CULTIVARS OF CHICKPEA (Cicer arietinum L.) S. Ali1*, S. Keywords: Agrobacterium,Acetosyringone, Cicerarietinum, Genetic transformation pool method of gene transfer (Sharma et al., 2002). Gene intensive, does not require sophisticated equipment, cost.

3 Sep 2004 Chickpea (Cicer arietinum L.), commonly called gram, Bengal gram, is a general concept to which chickpea is no exception... 2004; Sudupak et al., 2004) have provided many new approaches to.. 7.7.2 Conventional Breeding Efforts.. transformation and regeneration systems in plants have made it  Screening of Agrobacterium Mediated Transient ... Thus, Agrobacterium mediated genetic transformation may lead the solution. through genetic manipulation that would not be available using conventional breeding Moreover, regeneration and transformation frequency were explants wise Factors affecting transformation in chickpea were optimized by Akbulut and his  Regeneration studies in chickpea genotypes (Cicer arietinum L.) Chickpea (Cicer arietinum L.) is an important legume, which has worldwide conventional breeding Methods involving wild relatives of chickpea, but One of the prerequisites for successful genetic transformation is the protocol Compatible with in vitro plant regeneration method of target tissue2 . No of explants. Regeneration and transformation of an elite inbred line of maize

standardized for transformation and regeneration of chickpea, which otherwise was were subcultured every 2nd wk onto fresh medium containing 20 mg/L PPT. Keywords: Agrobacterium tumefaciens, chickpea, Cicer arietinum, genetic transformation, β-glucuronidase Conventional breeding is not very successful in.

30 Nov 2010 2010 Jul; 16(3): 273–284. in chickpea (Cicer arietinum L.) with an insecticidal protein gene: This may not be the complete list of references from this article. Altman A. High percentage of regeneration and transformation in chickpea. Brunstedt J. Sonication: a new method for gene transfer to plants. In vitro Regeneration and Propagation of Chickpea (Cicer ... A new method in With both cultivars there were no differences in rooting capacity between Key words: chickpea, Cicer arietinum; cotyledonary nodes; micropropagation; ble, this system should be suitable for genetic transformation as. COTYLEDONARY NODES AND MERISTEM TIPS OF CICER ARIETINUM L. cv. Recent Advances in Legume Transformation - Plant Physiology

23 Aug 2017 Plant Breed., 53, 2017 (4): 133–143 genotype-independent gene transformation method is of great interest in that hampered the progress of conventional plant efficient tissue culture regeneration systems is among facilitate gene transformation in plants that do not. Chickpea. (Cicer arietinum L.). Helicoverpa Resistant Chickpea Plants: From Bt ... - DergiPark 29 Jan 2017 3(1):52-60, 2017 Bacillus thuringiensis (Bt) chickpea plants expressing Bt genes as well as Chickpea (Cicer arietinum L.), a self-pollinating The conventional breeding approaches and chem- using modern genetic transformation technology to toxic to lepidopteran pests and non-toxic to humans. Plants | Free Full-Text | Plant Cellular and Molecular ... - MDPI 10 Jan 2019 A specific focus regards the progress in the genetic transformation of tree. as Ri T-DNA transformed roots could be regenerated into whole plants with a. that did not alter the fruit quality of grafted conventional sweet cherry scions [77]. transformation in the grain legume Cicer arietinum L. (chickpea). Rapid In Vitro Micro Propagation Of Chick Pea (Cicer ... Chickpea (Cicer arietinum L.) is an important grain legume of the Indian subcontinent, Since the conventional techniques employed in crop improvement may not keep Application of tissue culture techniques for genetic up gradation of Shoot regeneration from shoot apex/shoot tip is direct, relatively simple and is not 

8 Apr 2016 Chickpea (Cicer arietinum L.) is the second largest cultivated legume The phylogenetic tree was constructed by maximum-likelihood method in MEGA 6.06. Two of these genes were partial and four were non-WRKY genes... However, during transformation process, the number of shoots regenerated  Print chapter - IntechOpen

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26 Apr 2019 Chickpea transformation is an important component for the genetic Unfortunately, for most crops, transformation and regeneration remain This method is based on the Agrobacterium-mediated transformation of.. Transgenic chickpeas (Cicer arietinum L.) expressing a sequence-modified cry2Aa gene.

chickpea germplasm collections has not been extensively characterised due to a lack of complimentary genetic analysis approaches were used: genome-wide Rapid chickpea regeneration enabling the completion of three generations of.. haploid production in chickpea (Cicer arietinum L.): Role of stress treatments.

Shoot multiplication and regeneration of chickpea varieties: A) Shoot multiplication of Ein-Elbyda landrace on MS Cicer arietinum; in vitro; regeneration; thidiazuron; growth regulators. productivity of chickpea has not improved conventional breeding methods for improving engineering, and genetic transformation to.

Among leguminous foods, chickpeas (Cicer arietinum L.; CPs), also called.. As outcomes, the legume-based diet was the only dietary approach inducing a.. also referred as genetic modification, is an advancement from conventional plant could be transformed into non-IgE binding epitopes by single-site amino acid 

Optimization of factors affecting agro bacterium-mediated ...

23 Aug 2017 Plant Breed., 53, 2017 (4): 133–143 genotype-independent gene transformation method is of great interest in that hampered the progress of conventional plant efficient tissue culture regeneration systems is among facilitate gene transformation in plants that do not. Chickpea. (Cicer arietinum L.).

In vitro Regeneration and Propagation of Chickpea (Cicer ...

Chickpea (Cicer arietinum L.) is an important legume, which has worldwide conventional breeding Methods involving wild relatives of chickpea, but One of the prerequisites for successful genetic transformation is the protocol Compatible with in vitro plant regeneration method of target tissue2 . No of explants.

Chickpea (Cicer arietinum L.) is an important legume, which has worldwide conventional breeding Methods involving wild relatives of chickpea, but One of the prerequisites for successful genetic transformation is the protocol Compatible with in vitro plant regeneration method of target tissue2 . No of explants.

Regeneration studies in chickpea genotypes (Cicer arietinum L.)

Plants | Free Full-Text | Plant Cellular and Molecular ... - MDPI

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Screening of Agrobacterium Mediated Transient ...